Measuring interfacial and adhesion forces between bacteria and mineral surfaces with biological force microscopy
نویسندگان
چکیده
Interfacial and adhesion forces between living, unmodified bacterial cells (Escherichia coli) and mineral surfaces (muscovite, goethite, and graphite) have been directly measured in aqueous solution using a force microscope. Native cells are linked to a force-sensing probe that is used to characterize interactions as a mineral surface approaches, makes contact with, and withdraws from bacteria on the probe. Attractive and repulsive interfacial forces were detected at ranges up to 400 nanometers separation, the magnitude and sign depending on the ionic strength of the intervening solution and the mineral surface charge and hydrophobicity. Adhesion forces, up to several nanoNewtons in magnitude and exhibiting various fibrillation dynamics, were also measured and reflect the complex interactions of structural and chemical functionalities on the bacteria and mineral surfaces. Copyright © 2000 Elsevier Science Ltd
منابع مشابه
Mineral-Microbe Interactions Probed in Force, Energy, and Distance Nanospace
.......................................................................................................................................... ii Acknowledgements........................................................................................................................ iii Table of Figures ....................................................................................................
متن کاملCell adhesion of Shewanella oneidensis to iron oxide minerals: Effect of different single crystal faces
The results of experiments designed to test the hypothesis that near-surface molecular structure of iron oxide minerals influences adhesion of dissimilatory iron reducing bacteria are presented. These experiments involved the measurement, using atomic force microscopy, of interaction forces generated between Shewanella oneidensis MR-1 cells and single crystal growth faces of iron oxide minerals...
متن کاملAtomic force microscopy measurements of bacterial adhesion and biofilm formation onto clay-sized particles
Bacterial adhesion onto mineral surfaces and subsequent biofilm formation play key roles in aggregate stability, mineral weathering, and the fate of contaminants in soils. However, the mechanisms of bacteria-mineral interactions are not fully understood. Atomic force microscopy (AFM) was used to determine the adhesion forces between bacteria and goethite in water and to gain insight into the na...
متن کاملAtomic Force Microscopy Application in Biological Research: A Review Study
Atomic force microscopy (AFM) is a three-dimensional topographic technique with a high atomic resolution to measure surface roughness. AFM is a kind of scanning probe microscope, and its near-field technique is based on the interaction between a sharp tip and the atoms of the sample surface. There are several methods and many ways to modify the tip of the AFM to investigate surface properties, ...
متن کاملImaging Structures and Measuring Forces at Nanoscales with Atomic Force Microscopy
Zobrazovanie štruktúr a meranie síl na nanometrickej úrovni atómovo silovou mikroskópiou Interaction between interfaces in solution of chemical reagents is scientifically and technologically significant. Atomic force microscopy has been used to visualise structures of adsorbed surfactant molecules and measure interaction forces between surfaces with adsorbed molecules. This paper presents the r...
متن کامل